Botox is derived from a bacterium, Clostridium Botulinum, which is a naturally occurring bacterium that can be found in soils, forests, and lakes. It is also found in the gills and organs of crabs and various other shellfish, and in the intestines of fish and various mammals. Despite this, botulinum toxin has proven to be of value if injected into human beings in extremely minute concentrations.

It works by interfering with the contraction of the cells in muscles, which then lets them relax and become less stiff. Botulinum toxin is presently used to treat a number of different conditions, in addition to treatment for wrinkles and fine lines on the face. Among other things, Botox treatments in Harley Street are used for treating a variety of muscular disorders, excessive sweating, migraines, overactive bladder, neck, and shoulder muscle spasms, crossed eyes, spasms of the eyelids, and more.

It is also used, although not approved, for hay fever, cerebral palsy, over-production of saliva, anal fissure, and dysfunction of the throat which makes swallowing difficult. When used for treating frown lines and wrinkles it is applied to three main areas – the forehead, crow's feet, and frown. It has the effect of preventing the muscles from contracting and makes them relax, thus reducing the appearance of the lines and wrinkles. 

The treatment will last for up to three months before it wears off and another application is required. Subsequent injections have a tendency to gradually last longer, and so fewer treatments will be required. Botox treatments for wrinkles and facial lines are presently used by over six million people a year, most of them – but not all of them are women.